Why can I not detect other targets than GAPDH?

Hello, we're trying to detect Keratin 14 and 15 in Keratinocytes as well as FN-1 and CD90/THY1 in Fibroblasts from human skin. As reference gene GAPDH and Vinculin are used. In our first...

07 January 2021 6,652 3 View

Does anyone know instruments to measure "mental health literacy" in teachers?

Hi there, I am looking for questionnaires measuring mental health literacy in teachers and educators. does anyone know any studies in this area?

16 September 2020 1,682 4 View

Which solution do you use for low level actinide decontamination of laboratory surfaces like fume cupboards, work tops or floors?

Efficient decontamination of actinides like americium, uranium, neptunium and plutonium from laboratory surfaces. The decontamination should be easy in the sense that it could be performed on a...

03 June 2020 8,707 5 View

What is the best way to organize your MSD V-PLEX Human Proinflammatory Panel 1 raw data for analysis?

Hello People, does anyone have experience with the raw data analyses of the MSD V-PLEX Human Proinflammatory Panel 1? A friend of mine ran 4 plates and struggles with organizing the raw data for...

01 April 2020 3,566 2 View

How to do a non-linear regression analysis with multiple variables and a strong confounding variable?

Hi, I did outgrowth assays on spiral ganglion neurons from the inner ear with each supplemented by different concentrations of two neurotrophins. For each explant I did an image analysis and...

29 March 2020 8,703 7 View

Would somebody have suggestions for bioinformatic tools that predict changes in enzymatic (e.g. kinase) function based on changes in AA sequence?

Prediction of enzymatic activity based on common genetic variation.

19 February 2020 6,582 2 View

What are minimum and maximum values for ratios in Geopolmers (Si:Al, Na2O:H20, NaO:SiO)?

I am trying to perform a screening DoE to further study influence of a range of factors for example Metakaolin content on various performances like compression strength and to further optimise my...

23 January 2020 5,020 3 View

How to compare diagnostic efficacy of several methods in a within-subjects design?

Hi, I conducted a study on faking in personality tests, where the participants received two instructions (respond honestly vs. fake good) in a within-subjects design (oder was counter-balanced...

09 January 2020 5,182 3 View

Why do I get a different results (load-displacement-curve) when applying different loads in ABAQUS/Explicit?

I'm currently modelling a steel-concrete composite construction with headed studs in ABAQUS/Explicit and want to analyse the load-displacement behaviour of the headed stud. In the first case I...

18 December 2019 3,070 4 View

What amount of data is necessary to extract meaningful/significant Sequential patterns / rules in a sequential database?

10 March 2019 5,559 3 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

What are effects of steel jacketing on flexural strength?

Dear all, If steel jacketing is used to retrofit a reinforced-concrete (RC) column, can we use it to improve flexural strength? I understand that we leave gaps between the ends of the jacket and...

22 February 2021 1,906 5 View

How to combine two geometries in LAMMPS ?

I am trying to simulate contact angle of water on a quartz slab using LAMMPS. I have created and equilibriated the quartz slab and the water cluster separately. Now I want to create a composite...

17 February 2021 5,884 2 View

Is there any kind of data available for connection properties like signal strength between UE and RAN?

Is there any kind of data available for connection strength between UE and RAN? I am looking for signal strength, the geographical coverage of RAN's in a given Tracking Area, Coverage Area over a...

16 February 2021 1,073 5 View

How to mesh complex geometry?

I have been trying to mesh some complex geometry and have been having issues. I have attached images of the geometry in question. This structure is very large in size but has some comparatively...

05 February 2021 6,379 5 View

Can anyone direct me to an existing Validated Health Care Survey/Instrument?

Hello, I am working on a paper and looking for an existing Validated Health Care Survey/Instrument. I have been performing virtual ergonomic evaluations since the start of COVID and want to...

04 February 2021 4,756 2 View

Please recommend me the proper steps (blocking method) to generate Hexahedral mesh of the following geometry inside ICEM-CFD?

I am facing an issue while blocking for the geometry mentioned in the image. After blocking it ends with a triangular block due to its shape. How to create a hexahedral mesh for this geometry in...

02 February 2021 888 1 View

How to find nature of extremum of a functional? what are the mathematical prerequisites to understand it?

For a function, usually sign of second derivative (and if it is zero, even/odd index of higher order derivative whose numerical value is zero) is enough to detect whether the extreme point is...

25 January 2021 7,703 3 View

Frequency calculation with TDDFT???

Hi, I have been trying to calculate the frequency vibrations of the excited state S1 of an organic molecule (24 atoms). The geometry optimisation of the excited state converged smoothly howerver...

22 January 2021 1,463 3 View