Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Can Microplastics act as vector of chemical transport into human system on the model of stomach oil extraction ?

Chemical transport from micro-plastics to animal tissues has been reported in birds.

01 August 2019 9,216 1 View

Help with COMSOL Multiphysics?

Please can anyone help with COMSOL. I am trying to simulate gas storage in a porous media but I am at a loss on how to go about it especially how to input my mass balance, momentum and energy...

30 July 2019 1,453 2 View

I need more article on the assessment of nutritional status in school age children?

01 July 2019 6,210 2 View

How do You determine the ranges of value for your parameters, time and temperature using soxhlet extraction(N-hexane And Ethanol) for lemon grassoil?

I'm trying to conduct an experiment on the optimization of extraction of lemongrass oil using soxhlet extraction. My Optimum parameters are temperature, time and lemongrass to solvent ratio. I'm...

08 April 2019 9,256 1 View

Can I pool sera of chickens within a replicte together for LH FSH ELISA analyses in order to reduce cost of analyses?

I need to run ELISA analyses for reproductive hormones (LH, FSH) in chickens. The samples are however too many. Can I pool sera of birds within a replicate/treatment together for the analyses in...

19 August 2018 9,819 2 View

What is root relative squared error?

How to interpret root relative squared error with regards to prediction model of data mining? I have generated a data mining model using classification technique and the result shows that the...

25 February 2018 1,696 10 View

What are the main differences between C4.5 and Random Tree Data Mining Classification Algorithms in terms of generating decision tree?

I want know the main differences between C4.5 and Random Tree Data Mining Classification Algorithms in terms of generating decision tree?

22 February 2018 6,182 4 View

How do I plot my data using the Sigmoidal Langmuir equation?

I've included an image with showing the equation. I need to know what parameters to plot on the x and y axes. Any help will be highly appreciated.

15 January 2018 290 3 View

Which basis set is the most laudable for studying reactions involving halides?

23 November 2014 5,142 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View