How to identify products of tetra ARMS PCR with <20 bp difference?

More Pasha Ghazal's questions See All

What constraint can be used for rigid steel saddles and conrete beam under four point loading in ABAQUS?

Nodes are missing intersection with their respective master surface or outside the adjust zone, when using tie constraint.

01 February 2021 3,591 6 View

Why mutual inductor takes high current at no load condition?

I simulate an inductive wireless power transfer system in matlab. I set the primary and secondary mutual inductor resistance is 0.3ohm. So when i run the circuit it took high current at no load...

29 December 2020 9,536 3 View

Sterile chitosan folat polymer?

Hi, I want to start my cell culture to run a MTT assay , how can I sterilize my polymer?

15 December 2020 1,813 1 View

Solving chitosan folate nanoparticles in dmem?

Hi there, I have been struggling with solving my chitosan folate coated nanoseleniums in dmem culture media sincr it is only soluble in acetic acid , and hardly soluble in basic media. What can I do?

19 November 2020 5,636 3 View

TEM and SEM of chitosan coated nanoselenium?

Hi, I have a 25 ml sample of chitosan coated nanoselenium with average diametere of 112 nm, considering the fact that chitosan after being kept in water media swelles, how can I send a sample for...

08 November 2020 6,859 3 View

Addition of glycerol in measuring zeta potential?

Hi there I wanted to know if it is ok to add 2 or 3 drops of glycerol for suspending the nanoparticles in water before measuring the zeta potential?

26 October 2020 2,146 3 View

Chitosan coated nanoselenium ?

I'm running a MTT test for evaluation the effect of chitosan coated nanoselenium versus bare nanoseleniums, what are the controls for MTT assay? Should I test bare nanochitosans as well as...

27 September 2020 9,525 2 View

Should I do a quality assessment for studies that will be included in a systematic review, even if I am not doing meta-analysis?

The types of studies included are case reports, case series, epidemiological studies. The information collected will probably be analyzed qualitatively only.

17 August 2020 3,628 7 View

Is there a way (command or function or.....) to get the predicted value of output from fuzzy logic system, using matlab?

I want to get predicted values for output values related to some input values.

11 January 2019 7,539 4 View

How do install CFD OpenFOAM on Centos 6.4 on computer nodes (cluster)?

We are facing dependencies while installing CFD OpenFOAM software while installing it at cluster. We dont have docker? How do we manage it? Your valuable suggestions are welcome. Thanks in...

13 March 2018 1,279 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Péter Gyarmati

1. sequence - most reliable

2. PAGE - have higher resolution than agarose

3. if none of the above are available, try a high (>4%) agarose gel with 25/50 bp ladder and run the gel as long as possible (ie leave it running until the bands approach the end of the gel)

Boyang Huang

Péter Gyarmati what about the gel power set up? what voltage and current do you recommend?