It is not recommendable to dissolve the nanoparticles and to achieve 100%. If the particles are dissolve they will loose their nature and surface properties which is the key of the nanoparticles activity. So, take different concentrations according to OECD and mix them with the medium and proceed for cell vialibility assay. NRU assay will be a better assay for cell viability.
Thank you sandeep for your suggestion. I have tried this but the bhasma particles are depositing in the flask when added and kept for incubation. so how to go about this issue.
You're welcome buddy.. What's the average size of the particles. Mix it throughly for 15 min and then add to the culture. This will work for the particles of