I have tried growing it in different broths but everytime I try to scale up there is some problem with the culture and it takes almost 25 days to show the bioactivity so it takes a lot of time. If there are any suggestions on how to grow and eventually identify the bioactive compound I would be highly obliged.
Hi dear Subecha Rai I was wondering if you can tell me which culture media have you used and do you add antibiotics to the culture medium or not?
I have tried different media like CSPYME, GS, SS broth but it grows best in CSPYME. Also, since I'm looking for antibacterial property i have not added any antibiotics to the medium.
Have you used TSB medium? If you have not, I advise to try it cus we are using it with Shaking Incubator and the bacteria grow after around one week.
Yes i have but like I said the bioactivity is seen only after the 25th day and there seems to be some other bacterial growth in the 10th day or so whenever I try to do mass culture which is frustrating since it grows well in small volume.
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