Hello, i'm currently studying the effects of a methanolic extract against C. Elegans. I'm adding the extract with C. Elegans. But unfortunately, for every replication, the amounts of C. Elegans are different. How to get the same amount of C. Elegans for every replication? Thankyou🙏
Check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5222557/
Also check https://www.sciencedirect.com/science/article/pii/S0012160610012509
Dear Nadia Faradiba, Since there may be solid particles in the extract, the amount of ingredient you want to have in the amount of extract you take may be different each time. In order to take this to the fore, that is, to ensure that there are equal components in the extracts in equal amounts each time; 1) You can shake the extract vigorously and take a sample quickly 2) You can completely evaporate the solvent of the extract, divide the remaining solid into equal parts, then add methanol and dissolve it. I wish conveniences.
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