Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

What is exact impact of pH on preparation method of nanoparticles? why some nanoparticles are synthesized in ACIDIC pH while others in alkaline pH?

I am trying to prepare Cerium oxide nanoparticles. literature suggested the acidic pH of the reaction. what is the reason? what is the mechanism if so

28 November 2020 8,825 2 View

Estimate / Predict remaining useful life of a bearing without data of previous failure?

Hello, I am trying to write a program in Matlab to estimate the remaining useful life of a bearing without data of a previous failure. Do you think this is possible? I was thinking to use the...

27 November 2020 9,863 3 View

Two way ANOVA with non normal data?

Good afternoon everyone. I would like to know your oppinion or advise for the following: I would like to compare the results of a variable measured with a likert scale. I have two (or three)...

19 October 2020 4,564 4 View

Comparing two independent groups with very different sample size?

Hello eveyone. I would like to know your oppinion on the following. Do you think that it is adequate to compare two groups with very different sample size and non normal sample distribution? for...

19 October 2020 8,475 4 View

What is the recommended dose of sodium Bicarbonate intraperitoneal for the management of infusion pain on peritoneal dialysis?

Sodium Bicarbonate intraperitoneal dose for adult patients using PD solution containing of Lactate. How often can be used? And what are the Precautions should be taken during the administration

12 October 2020 7,567 1 View

Total Alkaloid Content?

Can someone suggest a simple calorimetric assay protocol for determination of total alkaloid content of plant extract?

10 September 2020 8,835 3 View

What are the environmental problems caused by phosphorus pollution ?

What are the environmental problems caused by phosphorus pollution ?

27 July 2020 948 34 View

What are the harmful effects of nitrates higher concentration on public health?

15 July 2020 3,900 10 View

What are the best methods of water management in the case of an agricultural drought ?

The importance of water resources is evident, and its management through all levels is imperative, especially in arid and semi-arid countries where water demand is highly required for irrigation...

10 July 2020 6,153 7 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

How to estimation of reverse-transcription on tissue?

I would like to evaluate the amount of reverse transcription (cDNA) on a 20um fixed mouse brain section. I am using random primers with RevertAid H Minus M-MuLV (reverse transcriptase). and would...

08 February 2021 1,957 4 View

How could a transcriptional factor purified as inclusion bodies (by using urea) exhibit a ratio 260/280>1?

I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...

05 February 2021 2,530 3 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

05 February 2021 292 3 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View

Why the western blot predicted band size of the MRTF-A is different between different manufacturers?

According to information from online database [MRTFA - Myocardin-related transcription factor A - Homo sapiens (Human) - MRTFA gene & protein](https://www.uniprot.org/uniprot/Q969V6) and [MKL1...

12 January 2021 9,492 3 View

What are the insilico tools available to recognize transcription binding sites on a promoter ?

I have to control the expression of a gene ( in vitro ). For designing an optimal promoter assay, I would like to know more about Transcription factor binding region, enhancer and repressor...

11 January 2021 7,992 3 View