How to get the more yield of RNA and Protein from Hamster buccal pouch tissues?

Kranthi kiran kishore Tanagala @Kranthi-Kiran-Kishore-Tanagala

12 September 2013 0 640 Report

We are using Mortar and Pestle for grinding the tissues. If any body has experience with tissue samples please send the protocol

Badges
Science topic

More Kranthi kiran kishore Tanagala's questions See All

Which MWCO filter to use to get rid of unwanted proteins?

I am working with snake venom, specifically targeting toxins around the 15 kDa range. I need to remove higher molecular weight toxins (above 20 kDa). Would it be advisable to use a 20 kDa MWCO...

05 June 2024 691 1 View

Which regression analysis will be applied if there are 7 nominal dependent variables and 1 ordinal independent variable?

which regression analysis will be applied if there are 7 nominal dependent variables(YES/NO categories) and 1 ordinal independent variable(3 categories: Low, normal, high)?

23 April 2024 9,971 3 View

What can be the alternate buffer for TAPS (PH 8.5) in Post tagmentation Wash step in Cut & Tag experiment ?

I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...

02 April 2024 4,214 0 View

How much of primary antibody do you add on slides in immunohistochemistry(IHC) / Immunocytochemistry (ICC)?

Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...

28 March 2024 259 3 View

How many number of moles of steam required to quench one mole of carbon monoxide(g) ?

Share the reference of number of moles of steam (practically / experimentally) required to quench one mole of carbon monoxide(g) ?

20 March 2024 8,316 0 View

How to label forest plots in RevMan 5.4?

While making forest plot Revman I label the plot as Left control and right experiment. I see that the data supports the control as the diamond is on the left. But when I change labels the...

25 February 2024 3,144 2 View

Grain size effects on antibacterial properties?

How grain size effects the antibacterial properties?

30 January 2024 6,225 1 View

How to separate dead cells from live cells when propagating suspension cells?

I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?

28 January 2024 8,818 6 View

Please Suggest me some techniques to Study Pollination and Breeding System of Dioecious Species in Field. Example: Baccaurea ramiflora?

Techniques Tools required Step by step Procedure

21 January 2024 8,960 0 View

What is your feeling in current system of education?

Education is imparting stress to each and every individual. There is heavy competition and all are striving to reach the limit. There are plenty of things to do and there is nothing in specific....

04 January 2024 9,726 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View