I add luminescence nanoparticles to the brain tissue. After analysis I found that I have too much non-specific binding. Would you please help me to get rid of these non-specific binding?
I have done three washing steps, each steps 5 min, with 1% BSA blocker.
You can conjugate specific biomarkers that over-express by the specific cells/targeting ligands or with an any external stimulus, such as pH-responsive charge conversion, temperature, magnetism etc. with your nanoparticles to target the desired tissues. You may see the followings:
Article PH-Responsive Charge-Conversional and Hemolytic Activities o...
https://pubs.rsc.org/en/content/articlelanding/2017/tb/c7tb01385j
Article Enhanced Blood Brain Barrier Permeability and Glioblastoma C...
Article Focused ultrasound delivery of Raman nanoparticles across th...
Article Nanoparticles for imaging and treating brain cancer
Instead of using BSA, Casein or othe protein blockers will thiols attach to your luminescence nanoparticles - if yes, then try https://www.prochimia.com/products/category/ultra-bioresistant-zwitterionic-ligands
Coating Surfaces with Zwitterionic Functionality Reduces Nonspecific Adsorption: See attached paper and link to commercially available thiol-zwitterionic ligands.
Article Zwitteration: Coating Surfaces with Zwitterionic Functionali...
Thanks a lot for your answers
I forgot to mentioned that the surface of my nanoparticles first modified with polymer and then conjugated with specific antibody Anti-ZnT3 which is specifically for ZnT3 antibody. However, I have more non-specific binding.
Now, please give me idea to get read of this non-specific bonding.
Thank a lot
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