Hi all, I am doing double marker IHC with anti Iba-1 (goat IgG) and MHC-II (Mouse IgG2b) antibody in frozen section of human retina. In my single marker IHC, both antibody stains different cell populations as expected but in double marker IHC, both are staining the same cell populations. Does anyone have any idea how can I overcome this problem?

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