The problem is before subculturing the inoculated broth had to be kept in the fridge since it's not possible to do the experiment after 12 hrs. Also it is taking more time to reach log phase so we are missing on the exact 0.3-0.4 od and it exceeds
The transformation efficiency, genetic markers, packaging formats that fit your workflow are the most important considerations when choosing competent cells for your cloning experiment. Each of these factors will directly impact the time and effort required for your project, as well as its success. There is also need consider number of cell types and formats. The factors and the attributes inherent in each competent cell line. Exploratory studies will help you to make the right choice to achieve your cloning goal
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