I am working on the identification of sex pheromones of moths, I have collected the pheromone gland extraction using hexane, i have got bio-active compound in GC-MS, but the peak of the compound looks too much contaminated with other materials, i can't read the fragment ions very clearly, and can't make sure which fragment ions belongs to compound and which are from impurities, I have tried average across the peak and and background subtraction, though i can't minimize the impurities, is there any other way of purification, separation to get clear mass spectrum through any software? Your suggestion and answers will be highly appreciated, Thanks
Re-extract analyte using n-hexane several times to improve on degree of purity of compounds before running on GC-MS
Valentine Mbatchou hexane used as solvent was purchased by a company and it was HPLC standard grade hexane and purity percentage was greater than 95% so it was n-hexane,, thanks for your kind suggestion
Hello. So, something else is co-eluting with your compound of interest, right ? If you have a column with a slightly different polarity that might help. If not, decreasing the heating rate could also improve the resolution.
Good luck.
Vanderson Barbosa Bernardo you are absolutely right, either it is isomer of my interest compound which is co-eluting or the impurities, your suggestions are really valuable for me, though i have not an other column but i can decrease the heat rate to improve the resolution, appreciated
Have you tried using a devolution programme such as AMDIS from NIST (free download). Explanation can be found in:
https://www.agilent.com/cs/library/applications/5990-5052EN.pdf
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