Failure of protein expression ?

Dear all, is there any possibility that C-myc epitope tags at recombinant protein can cause the protein degradation/failure in protein expression ? I can't find any previous literature that can...

11 August 2020 7,376 4 View

Why is Legionella spp. from environmental samples cultured in a CO2 enriched atmosphere?

I've recently started working in an environmental analysis laboratory and wondered why is it that, in order to detect Legionella spp. from water samples, the incubation is done in a closed bag...

23 July 2020 503 4 View

Unable to express Recombinant Protein in Pichia Pastoris?

07 July 2019 3,109 6 View

Which of the journal article publication has a more academic weight between the empirical and review article?

Lecturers in a college of education argued on which article has the higher points between the empirical and the review article. Some think it is better to engage in empirical writing rather than...

29 November 2018 8,689 8 View

How to extrapolate result from in vitro (ug/mL) to in vivo?

I have obtained result from in vitro which 10 ug/mL. How to predict what value of doses we need to use in the mouse (mg/kg). Is there any specific calculation to extrapolate the data to mg/kg? or...

01 February 2014 7,878 12 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

How to assure whether a regression tree overfit or not by seeing bias-variance value of the model?

Lets consider a regression tree in which variance is 1.1065*e-10 and bias is 2.962e-13. Also the model RMSE on training set is 1.5e-5 and on training set is 1.2950e-5. Is the model is overfit or...

22 February 2021 7,755 2 View