I want to determine the number of a certain gene family according to genome information. Any instructions will be very appreciated.
You can go through the uniprot web site and search the protein coded by your specific gene....For this protein, many genes will come up in several pages...
Freezing stress can induce water loss due to the fact that formed ice breaks the membrane and electrolyte flux out of cell. However, chilling stress can also cause water loss.several reports...
07 August 2016 3,930 4 View
I want to treat fruit with NDGA, but according to references that NDGA was soluble in water with detergent of Tween20 (0.05%). indeed, I tested it and found it is still unsoluble. Why, who can...
08 September 2015 369 0 View
I want to use NDGA solution to treat fruit. But it is insoluble in water, how to dissovle NDGA ? I have found it is soluble in ethonal, DMSO, but ethonal properity is harmful to fruit, and effect...
05 June 2015 9,306 2 View
Does anybody knows a website for free online statistic analysis? Local statistic softwares are not very convenient for lots of small datasets, free online statistic analysis is necessary for...
04 May 2015 9,061 3 View
I want to evaluate the purity of plasma membrane-enriched fractions isolated in our laboratory by immunoblotting with antibodies raised against known subcellular membrane (endoplasmic reticulum,...
11 December 2013 3,169 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View