How to understand chilling-induced water deficit in detached horticultural products under chilling condition?

Freezing stress can induce water loss due to the fact that formed ice breaks the membrane and electrolyte flux out of cell. However, chilling stress can also cause water loss.several reports...

07 August 2016 3,847 4 View

How to solublize nordihydroguaiaretic acid (NDGA) in water ?

I want to treat fruit with NDGA, but according to references that NDGA was soluble in water with detergent of Tween20 (0.05%). indeed, I tested it and found it is still unsoluble. Why, who can...

08 September 2015 305 0 View

How do I dissolve nordihydroguaiaretic acid (NDGA)?

I want to use NDGA solution to treat fruit. But it is insoluble in water, how to dissovle NDGA ? I have found it is soluble in ethonal, DMSO, but ethonal properity is harmful to fruit, and effect...

05 June 2015 9,231 2 View

Anybody knows a website for free online statistic analysis ?

04 May 2015 9,000 3 View

Which subcellular membrane markers should be chosen to test purity of plasma membrane by immunoblotting? where can I buy them?

I want to evaluate the purity of plasma membrane-enriched fractions isolated in our laboratory by immunoblotting with antibodies raised against known subcellular membrane (endoplasmic reticulum,...

11 December 2013 3,071 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View