I am not sure what you mean by "value of lambda 1 and 2".
based on absorption, you need to know what the absorption co-efficient of the materials is, at a given wavelength, and then work out your unknown thickness from the Lambert-Beer law. Away from the absorptions, if you can see interference fringes in the baseline, you can fit these to get a thickness, if you know your material refractive index.
The attached paper teach you easily how to calculate film thickness and the refractive index from the transmittance as a function of lambda using Swanepoel method.