I am looking for a standard protocol for determining the Total Alkaloids in Tobacco Leaf by spectrophotometry. All papers on this topic don't have detailed information and some critical information in their followed procedure completely missing.

I wish I can find a database for plant phytochemicals and for plant Phyto-constituents.

For molecular Biology, so many manuals and step-by-step protocol for different purposes easily find, but for phytochemical analysis, such types of protocols do not exist.

Thanks.