How to find out reaction mechanism of formaldehyde and ammonia via DFT study?

More Gaurav Jain's questions See All

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

What binder would be the best choice to modify glassy carbon electrode with a 2D Material like Graphene or MXene?

I am trying to drop cast 2D Materials on Glassy Carbon Electrode for Cyclic voltametry and EIS purpose. What would be best binders to use?

23 July 2024 5,776 2 View

Sample size for qualitative study?

I am working on scale development in behavioral finance by undertaking a mixed-method approach using the exploratory sequential design. The phenomenon has diverse meanings in existing literature...

20 July 2024 9,153 11 View

I am carrying out an experiment on Trichoderma. How can I measure OD of the fungal growth after 7 days?

I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....

11 July 2024 6,251 1 View

Does Nature Scientific Reports waive open access fee for industry authors?

I came across the Green Building and Sustainable Architecture collection under Nature Scientific Reports some weeks ago. https://www.nature.com/collections/gajghaebce The special issue/collection...

10 July 2024 5,533 1 View

I need help with cloning a 200 bp insert and a 4,7 Kb pEGFP-N1 vector. How can I do it?

I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...

09 July 2024 4,277 1 View

Can I do Parallel Analysis for Principal Axis Factoring Method?

Recently I was suggested to do Parallel Analysis and compare with EV>1 to determine no of factors for my scale. However, the scale I have developed uses Principal Axis Factoring and not PCA. If...

30 June 2024 1,813 2 View

How to reduce the permeability of pdms?

I want to trap fluid (water..) in the PDMS channel for a long time. After a short period, the channels become empty of fluid. Is there a way to reduce the permeability of PDMS?

17 June 2024 2,703 4 View

How can I draw curve wavelength vs EQE for fixed current of a LED in silvaco tcad. But tonyplot is showing anode voltage instead of wavelength ?

silvaco-tcad

25 May 2024 9,599 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

Ajay Khanna

Dear Gaurav, you just asked a question answer to which everyone else is looking for because it is not a straight one. Just to give a glimpse on how to proceed please follow the link below, its a video tutorial by IaNiusha YouTube Channel, about: "HOW TO OBTAIN A FULL REACTION PATHWAY of Menshutkin Reaction (https://en.wikipedia.org/wiki/Menshutkin_reaction)"

https://www.youtube.com/watch?v=qrL_yWkJj-Q&index=10&list=PLxm4o04Xz3BZtX2MljVUpjapUYegn0a3A

Regards

Gaurav Jain

Thank You. I will study it and check it.

Regards,

Mohammednoor Altarawneh

I would start from a well-known mechanism by repeating some literature work

Abd Al-Aziz A. Abu-Saleh

This paper might be helpful doi.org/10.1016/0166-1280(93)87223-Z .

Good luck,

Thank you Sir for this reference