Can you help me to find the concentration in %? I used 100mg sample in 10mL of 80% ethanol for the carbohydrate extraction. The mixture then centrifuged and decanted into beaker. The final volume was made up to 25mL by adding distilled water. For the analysis, standards were made by:
adding 0.1g glucose into 1L distilled water. Then, 1.0mL, 2.0 mL and 3.0mL of the solution was added to test tube and made up to 5 ml with distilled water.
Glucose standards (mg/tube)
0
0.1
0.2
0.3
5mL of the 25mL sugar extract was transferred to a 100mL volumetric flask and made to volume. 5 mL of this diluted sugar extract and the standards were put in ice and added with 10mL of anthrone. Then the absorbance at 630 mu were measured.
What made me confused is that the standards used in the protocol is mg/tube. So how to get it in %. Thank you in advance.
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