Actually i treated silver nanoparticles with Streptomyces sp. i want to know where it is deposited and i also want to find any mutation in Streptomyces.
Have you considered if the Streptomyces is able to reduce it to its ore form (liquid) which may still be in the vicinity of the medium. The Streptomyces may mutate when you do electric deposition of the silver into the microorganism.
Metal analysis of Streptomyces before and after will let you know if the silver is deposited in the microorganism.
Nice Research, Good luck.
I guess you want to know if the nanoparticles are on the surface or within the cell, right? If so, what is the charge of the nanoparticles under your studied conditions? What is the charge of the cell surface? Is there some kind of hydrophobic protection on the surface of the nanoparticle?
Yes Prof.Omar. I purchased NP from sigma - 1o nm. it contains sodium citrate as stabilizer.
Bacteria is negative charge.
If it is just a matter of surface localization, I think SEM or TEM could give best results. AFM is also good if the NP sizes are bigger.
Please check out this project for further inspiration:
https://www.researchgate.net/publication/308659360_Coupling_of_radiofrequency_with_magnetic_nanoparticles_treatment_as_an_alternative_physical_antibacterial_strategy_against_multiple_drug_resistant_bacteria
Regards,
Subramaniyam
Article Coupling of radiofrequency with magnetic nanoparticles treat...
nanoparticle size is 10 nm. The concentration is 0.02 mg/mL. i added only 50 micro liter. After this treatment, know i want to locate this nanoparticles. i think fluorescent microscopy may help but how to stain nanoparticles and live and dead cells......
If you can do confocal microscopy, you can check the live/dead viability using SYTO and propidium iodide:
http://aem.asm.org/content/75/9/2920.full
dear subra, i want to differentiate live, dead and nanoparticles........after treatment.....
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