Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is iron oxide (rust) a problem in DNA extractions?

Hello, I just started extracting DNA from some spiders, using 5-mm ball bearings to grind the specimens in a tissue mill. I had ordered the ball bearings on Amazon, and thought it was a good idea...

21 July 2020 2,271 6 View

Comparing two variables to one variable- is this possible in SPSS?

In a quantitative study, three variables-- I am comparing two variables (self-concept & well being) with another variable (intent to leave profession). I am predicting to see a positive...

05 May 2020 6,362 3 View

Is there a human gut microbiome database that shows normal variability as well as pathological vs normal ranges for specific microbes?

When a diagnostic lab furnishes normal ranges for certain microbes, is there any place to compare their ranges to what scientists are discovering is normal variability or adaptations? Is there a...

29 December 2019 4,771 2 View

Is it imperative in psychology research to state the null hypothesis?

I have had feedback from a reviewer that I need to restate my hypotheses as null hypotheses. My supervisor disagrees with this suggestion. Having done a bit of digging, I found Szucs &...

12 December 2019 1,447 4 View

How to fit data with binomial distribution?

Hello researchers, Which program or software works best to fit data with binomial distribution? Many thanks, Neena

15 November 2019 6,609 3 View

Stereochemistry Check for Peptides?

Hello researchers, Is PROCHECK the correct tool to check the stereochemical quality of small peptides? My peptide of interest: polypeptide with 16 alanine residues. Many thanks, Neena

28 October 2019 4,653 2 View

I have a GST tagged fusion protein in soluble phase will it have folded?

Normally this protein EIF4E is expressed untagged and expresses in inclusion bodies that are refolded through dialysis, but its very long winded and likes to precipitate during dialysis resulting...

11 June 2019 622 3 View

I am in the process of recruiting participants for my PhD using IPA and am now re-thinking the data collection design. Can you give me any advice?

28 April 2019 8,737 4 View

Approx. how much IL10 do activated T cells secrete after 48 hours in culture?

My students are culturing mixed mouse splenocytes in culture for 48 hours with anti-CD3/28. We are then going to analyse for the presence of Tregs and measure IL10 secretion by ELISA. In order to...

20 March 2019 5,857 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View