What are some good polymer to use for packaging a biological product ,which can be steril by gamma?

As different polymers with different characteristics are exist,I want to know What polymers can be used for packaging an orthobiologic product(bone allograft),which can have enough resistent to...

22 December 2020 8,196 5 View

For LDH activity, is there any difference to add NADH, pyruvate and Tris separately in the well or prepare a mixture of them and then add?

Hi everybody, For LDH enzyme activity, I prepared to different series of buffers based on two protocols: 1-I prepared the solutions of 100 mM Tris, 3.6 mM Sodium pyruvate, 3.4 mM NADH...

22 October 2020 9,855 3 View

Nursing article about corona virus?

Hello Dear I need to catch scholarly articles about people attitude about contagious diseases like corona virus and how to mange their fear despite of maintaining their sensation about it

23 March 2020 6,936 12 View

Thin films of tio2 is made with sol gel (dip-coating) I want to know why when thin film thickness of Tio2 increases, anatase change to brookite?

Thin films of TiO2 is made with sol gel (dip-coating) I want to know why when thin film thickness of TiO2 increases, anatase change to brookite?

26 April 2018 2,142 4 View

Can anybody help me about removing of endotoxin from bacteriophage?

Hi. How can I remove endotoxin from bacteriophage lysate with a simple and high-efficiency method?I will appreciate to help me.

17 October 2016 4,544 1 View

How can I get the clear and high-quality image of my cells have been transformed by GFP vector under florescent microscope?

06 September 2016 6,924 4 View

When results of otsu method is better?for more levels or lower levels?

17 June 2016 1,571 3 View

Hybridoma cell cultures with HT medium?

Dear my colleages I am culturing hybridoma cells about 3 weeks. Now They are in the T-flask. I don't know how many times I must culture them in HT Medium?

27 February 2016 5,245 3 View

Which thresholding techniques are better if our image has Intensity homogeneities?

27 October 2015 9,872 17 View

I am looking for SAS software code for Augmented Design. Can someone help me out?

30 January 2015 2,107 18 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View