08 November 2022 0 7K Report

How can I recognize the possible antibodies and also the best antibody for a specific antigen?

( as I want to choose a proper antibody in immunobiosensor to detect Alpha defensin )

More Atefeh Shiri's questions See All
Why my pcr bands are fuzzy?

Hi Although the ladder is sharp but my pcr bands are too fuzzy and like smears. I try to set up one of my genes but it still stands for 3 bands, unfortunately. Tm is 65 but there are still...

17 June 2024 7,412 1 View

Do we have to do all our cellular tests by treating with ic50 con?

Hi, I have done mtt test and realized that my reagent ic50 is 50nM. Do I have to do ally cellular assay by treating with this concentration? Do we do the mtt test for this purpose? Of course I...

10 May 2024 1,314 2 View

How to determine hydrogen peroxide concentration in a sample?

Actually as I searched about, there are two major method in determining hydrogen peroxide concentration, one is titration with sodium thiosulfate and other is titration with permangenate, I wanted...

29 April 2024 3,565 4 View

Where can I get a sinogram CT scan of the brain?

I am a graduate student writing to humbly request your assistance. I am currently working on my thesis which focuses on medical image reconstruction techniques. For my research, I need real...

27 September 2023 1,794 3 View

What is the best protocol of RNA( lncRNA) extraction using trizol reagent from plasma ?

I used this protocol but I did not answer 1) add 250 µl plasma to 750 µl Triazole 2) 5 min incubation 3) add 150 µl chloroform, mix 12 sec, 3 min incubation 4) Centrifuge at 10,000g for 10 min at...

11 August 2023 4,191 0 View

Why do I have smear in my PCR product loading on the gel (1%) for both the test samples and the water control (DW)?

I have set up a PCR using primers that amplify only the exons of my gene of interest. The template I am using is cDNA (mouse samples). I set up this PCR a year ago with an annealing temperature of...

30 July 2023 256 15 View

What is the wavelength of Glycerol monostearate maximum absorption in spectrophotometric analysis?

in order to quantify the concentration of Glycerol monostearate in aqueous solution by UV spectrophotometer, what is the maximum lambda of Glycerol monostearate?

13 May 2023 4,307 3 View

I have some questions about the culture of activated Platelet?

I work on Platelet and need to some information for culturing. How can activate platelet I mean which activator and how much time? as well as how can be stop this process without platelet...

08 May 2023 2,897 0 View

Image reconstruction code request‏‏‏‏?

Hello, I'm currently working on a project in lesion detection and I'm looking for image reconstruction codes for algorithms related to this field.(ASIR. 3DRP. FORE FBP. FORE OSEM. IMR or SAFIRE) I...

19 April 2023 2,712 2 View

Image reconstruction code request?‏‏‏‏

Hello, I'm currently working on a project in lesion detection and I'm looking for image reconstruction codes for algorithms related to this field.(ASIR. 3DRP. FORE FBP. FORE OSEM. IMR or SAFIRE) I...

19 April 2023 9,065 0 View

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Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

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What procedure should I follow for current controlled PPy film electrochemical deposition on ITO?

For example, I perform passivation process before electropolymerization of Py for adhesion to titanium surface. Is a similar or different procedure required for ITO? If necessary, I would be glad...

06 August 2024 3,176 0 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

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How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Dimensions of an MJ Research 96-well alpha module?

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Ti6Al4V - Phase differentiation between alpha and alpha prime martensite?

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How to calculate conductivity using Electrochemical Impedance Spectroscopy's Nyquist plot?

Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...

29 July 2024 1,376 0 View

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