I need to obtain essential oils from in vitro plants but they are very little still. I know microwave extraction can be used for small samples. Is there another cheap method?
Dry the sample in a 105 Celsius conventional oven overnight. Weigh and grind the sample. Accurately extract ~0.5 g in a Soxhlet unit using Diethyl or Petroleum Ether (not Hexane). Air dry the extract and weigh the amount of oil. Calculate the amount of oil as is or dry basis.