Sample size calculation?

07 September 2020 1,318 4 View

When can E.coli secrete most of the extracelluar protein?

Hello, I'm doing optimization of an extracellular protein cloned in the pET26b vector along with PelB leader sequence. I want to know the best time at which cells can secrete most of the protein...

13 October 2019 5,915 6 View

How to confidently predict the 3D structure of a protein?

I have tried to predicted the 3D structure by using multiple online softwares like I-TASSAR, MUSTER, Swiss-Model and (PS)2 but predicted 3D structure from each software has bad quality and can not...

27 November 2018 9,085 3 View

How to do molecular dynamics simulations for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio??

Hello, I want to do simulations analysis for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio. Kindly suggest me a protocol. Thanks

11 February 2018 9,627 3 View

How do you calculate the total phenolic contents in a plant extract sample?

I have to find out the total phenolic contents in five different plant extracts. I have read many papers but am unable to understand the calculations how to express total phenolic contents as mg...

26 November 2014 5,255 56 View

Can anybody suggest a suitable region to design porcine specific qPCR assay, apart from mitochondrial genome?

I want to design species specific primers and probes for porcine, bovine, ovine, chicken etc, as mitochondrial genome is really conserved it's really difficult to design assays from MT genomes....

05 August 2014 8,873 3 View

What is the exact mechanism of selection of an X-chromosome for inactivation during female embryonic development?

It is said that a default state of the X-chromosome in female mammals is inactive and one of them is activated, as in case of females with three or four X-chromosomes, i.e. all except one remain...

26 September 2013 7,614 2 View

Can Anyone tell me how can we plot data for TGA curve through the data? i want to plot mass % v/s Temp but i dont have any data for mass %?

01 January 1970 6,946 3 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

How to extract NLR genes from a database?

Greetings to all, I am not a bioinformatics person, but looking for a method that help me extract all NLR genes from benthamiana, tomato and soybean databases.. any suggestions so I can use my...

08 February 2021 4,557 2 View

What should my objective ?

I am going to start my master program (msc agri hons) research in rull of potassium in tomato vigetable crop so what should my objective please guide me Thanks

13 January 2021 4,635 6 View

The use of Orange, a high level tookit for Machine Learning, data mining and data science for scientific research or just teaching?

Related to data mining/data science, I recently discovered Orange (https://orange.biolab.si/) a high level very powerful free toolkit. Using it is very simple and you can test different models in...

09 December 2020 8,832 4 View

Tomato pollen internal membrane dye?

Hello everyone, I wonder if you can help to suggest any internal membrane dye that would enable me to stain the generative cell in tomato? unfortunately I have no marker tagged to the cell but I...

28 October 2020 2,335 3 View

Has anyone extracted RNA from tomato fruit exudates? How do you proceed to collect the exudate from the fruit?

I have technical doubts about how tomato fruit exudates are collected. Does anyone know any protocol or article that details how to collect it?

22 October 2020 9,743 2 View

How can I get help to identify a species of Coniopterygidae from Peru?

I found a species of Coniopterygidae preying on eggs and nymphs of Aleurothrixus floccosus in a lemon tree. I have followed its biology and have pictures of eggs, larvae, pupae and adults. With my...

16 October 2020 6,421 2 View

Does citrus plant can be effective as Nanopesticides?

good day everyone may i ask a request if there are any study about citrus plant that been used as nanopesticides since google only show a nanoformulation and nanoparticles that uses citrus plant....

14 October 2020 6,898 4 View

How to rank data and transform responses of a variable in SPSS?

So i have data something like this in SPSS at the moment: case 1: orange, bananas, mangoes case 2: bananas, oranges, mangoes So the first respondent ranked orange as best fruit whilst second...

07 September 2020 8,305 5 View