Could anybody please help me with some simple/easy methods to extract beta carotene from tomatoes. new to this area..
Thanks in advance.
Carotenoids are thermolabile (xanthophylls); their heating being indicated only when it is absolutely necessary. The separation of carotenoids is done at room temperature or up to –20°C, in the dark. In the case of hot saponification they should be protected by a low–boiling solvent (30–60°C). Carotenoids may be oxidized in the presence of oxygen or peroxides, because of their sensitivity to oxygen in the adsorbed state (in thin layer or column chromatograms). It is necessary to operate in inert conditions (under nitrogen or vacuum). The oxidation during the extraction and saponification can be minimized if it is carried out in a nitrogen atmosphere. The exposure of carotenoids to acids, leads to changes such as: the oxidative decomposition, cis–trans isomerization and isomerization of 5,6–epoxides and 5,8–epoxides. The inconveniences are minimized by neutralization (calcium carbonate, pyridine, dimetilalanine). It works with purified solvents, freshly distilled, chlorinated derivatives (dichloromethane or solvents containing hydrochloric acid).
Source: https://www.omicsonline.org/open-access/methods-of-analysis-extraction-separation-identification-and-quantification-of-carotenoids-from-natural-products-2157-7625-1000193.php?aid=77160&view=mobile
Carotenoids are thermolabile (xanthophylls); their heating being indicated only when it is absolutely necessary. The separation of carotenoids is done at room temperature or up to –20°C, in the dark. In the case of hot saponification they should be protected by a low–boiling solvent (30–60°C). Carotenoids may be oxidized in the presence of oxygen or peroxides, because of their sensitivity to oxygen in the adsorbed state (in thin layer or column chromatograms). It is necessary to operate in inert conditions (under nitrogen or vacuum). The oxidation during the extraction and saponification can be minimized if it is carried out in a nitrogen atmosphere. The exposure of carotenoids to acids, leads to changes such as: the oxidative decomposition, cis–trans isomerization and isomerization of 5,6–epoxides and 5,8–epoxides. The inconveniences are minimized by neutralization (calcium carbonate, pyridine, dimetilalanine). It works with purified solvents, freshly distilled, chlorinated derivatives (dichloromethane or solvents containing hydrochloric acid).
Source: https://www.omicsonline.org/open-access/methods-of-analysis-extraction-separation-identification-and-quantification-of-carotenoids-from-natural-products-2157-7625-1000193.php?aid=77160&view=mobile
Please take a look at the following links.
https://pdfs.semanticscholar.org/8240/5c5a24a1d76d29a1bca0e1137c03199e17db.pdf
http://kau.diva-portal.org/smash/get/diva2:506614/FULLTEXT01.pdf
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