CRISPR is a technology that can be used to edit genes and, as such, will likely change the world. The essence of CRISPR is simple: it's a way of finding a specific bit of DNA inside a cell. After that, the next step in CRISPR gene editing is usually to alter that piece of DNA.Imagine a future where parents can create bespoke babies, selecting the height and eye color of their unborn children. In fact, imagine that all traits of life forms can be customized to one’s preferences: domestic pet size, plant longevity, and more.

The zebrafish has emerged as a leading model organism for the study of vertebrate biology, because of the remarkable cellular resolution with which the embryo can be studied, the ease of assaying its development and physiology in the laboratory, and its amenability to genetic analysesThe zebrafish is a powerful experimental system for uncovering gene function in vertebrate organisms. Nevertheless, studies in the zebrafish have been limited by the approaches available for eliminating gene function. Here we present simple and efficient methods for inducing, detecting, and recovering mutations at virtually any locus in the zebrafish. Briefly, double-strand DNA breaks are induced at a locus of interest by synthetic nucleases, called TALENs. Subsequent host repair of the DNA lesions leads to the generation of insertion and deletion mutations at the targeted locus.

https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1002861

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613589/