How to explain reverse time resolved emission spectra in certain proteins?

19 October 2022 1 4K Report

Is it possible to get the reverse TRES spectra in TRIS buffer?

Dear Anjali,

I think (but I'm not sure) in your case the protein is already in excited state (unstable state). So when you give absorbance of particular wavelength it excites to higher state. During emission spectra, it might give lesser wavelength. Please refer following attached case:

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