I have liquid plant extracts, extracted in water, and need to evaporate them to get dried extracts, but we don't have rotary evaporator. Any ideas or protocols?
Incubation in 30 or 40 C with shaking, does it work?
Thanks in advance.
You could try with a magnetic stirrer and hot plate around 35 - 45°C putting your extract on a porcelain capsule. If you do this be extra careful when the extract begins to loose water. Keep the stirring and temperature gentle as some labile components may be lost or worse, you could end up burning the dried extract rather easily.
Good luck
which solvent you are using ? if its chloroform or hexane then it will be easy to keep in air for some time for the evaporation of solvent .
Regards
1.HOW DID YOU EXTRACT WITH WATER...AT COLD / RT OR HOT CONDITION (TO KNOW IF YOU HAVE LOST ANY ACTIVE THERMOLABILE COMPOUNDS).
2.WATER EXTRACTS CAN BE CONCENTRATED (UNDER VACUUM ...OPEN DRYING TAKES LONGER TIME AND CAN DEVELOP FUNGUS ) AND THEN DRIED ON SS PLATES (NORMALLY WATER EXTRACTS CONTAIN GUMS/ RESIN MATERIALS AND IF DRIED ON GLASS/PORCELAIN PLATES THEY ARE VERY STICKY AND YOU MAY HAVE TO BREAK GLASS TO SEPARATE WHILE SCRAPPING. HENCE BETTER USE SS PLATES FOR FINAL DRYING.
Razan Marouf Your best option with a water extract is a freeze dryer. I hope you have access to it.
B.k Chakravarthy , thank you for the explanation.
1- the extraction was conducted at hot conditions (boiling for 5 minutes).
2- I agree with you, so I will try magnetic stirrer on hot plate, placing the extract in a side-arm flask, then applying vacuum.
Ayorinde Victor Ogundele
I thought about it but unfortunately i don't have access to it.
Thank you.
I performed the work through Incubator apparatus in 25-30 Centigrade degree within six month ago but i did not get the correct and rational result. I think that it need to pre-treatment or maybe post-treatment. I am studying on it with my supervisor for a research item.
The best way to handle water extract is to use freeze dryer or rotary if these are not at reach. evaporate by heating at temperature of 45 degrees which will not affect thermolabile constituent of medicinal importance.
Thank you all for the helping answers.
Freeze drying (lyophilization) and rotary evaporator are the best choices, but I have no access to them right now.
As mentioned by B.k Chakravarthy, open drying takes long time and can develop fungus, and I also think heating at 45C or 30C will have the same probability.
In my opinion, evaporating water extracts should be done in minimal time to avoid any change in the active compounds or development of fungus.
So, I think I will start with magnetic stirring with applying a vacuum, the best available method for now.
Regards.
yes, the incubation replaces the rotavap but the temperature should be less than 40 to provide dégradation of composition.
Note: the best way to évaporate the solvent it's the rotavap or lyophilizer.
Fairouz Boubrik
Thank you for the contribution.
I'm not fully agree with you that incubation replaces the rotavap even below 40C, I don't think you can get the same result as in the rotavap (as mentioned by Hamid Reza Farhang)
"Note: the best way to évaporate the solvent it's the rotavap or lyophilizer."
then which ways do you think are the best? and why these are not?
If you have experience with similar projects or literature concerning this, please share it with us.
Waiting for you explanation and thanks in advance.
Hello,
My advice is to find a partner lab in the city where you are to do these experiments properly specially you are attending to publish the results afterwards.
In Kinshasa where I did my Bachelor studies I had at that time only one or two lyophilizers to serve all scientific community.
Best of luck,
Jean-Pierre.
I think to try extraction with solvents, it can work
Good luck
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