Hello everyone, I have a crude extract and I did MIC for it, it gave a minimum inhibitory concentration of 1 mg/ml. Later, I did HPLC for that crude Extract and the concentration of it inside the vial was around 500mg/ml, and I got 9 peaks from it.
The LC condition that was used:
Mobile phase:
Column: C8
The flow rate: 0.7ml/min, with an injection volume of 100ul. I used the isocratic method of 95 (water)/5 (acetonitrile).
The peaks were collected in different tubes, and I want to evaporate the acetonitrile and formic acid and then do the MIC for each individual peak that I collected, and I am not sure how to evaporate acetonitrile and formic acid, and once I evaporate them how many ml or ul of water should I dissolve my peaks with?
Your suggestions are highly appreciated, Thank you.
The best option is to evaporate the different tubes under nitrogen stream or let it dry by itself but since you have high contents of waters it would take forever. You can use some temperature in order to speed-up the process of evaporation.
You can use a centrifugal concentrator (Speed Vac or MiVac) that allows evaporation of hight water content samples.
You could use a freeze dryer or a rotary evaporator, depending on what equipment you have in your laboratory.
Thank you so much Gabriela S. Barragan-Zarate , Sarah Ployon , Andreu Fabregat Rossell
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