I want to count the number of red, blue & green pixels (or those in CMYK system) within the area of the polygons, which correspond the spots on leaves (please see files added). So what is the simplest software (maybe Photoshop, Origin, AutoCAD, ImageTool (released by UTHSCA) or other?) to cope with this task? And what is the stepwise algorythm to perform this task? It would be better (as for me) to get the Application, which is free and Windows10+ compatible. Although ImageTool 3.0 is good tool for the polygon area measurement, but, unfortunately it isn't Window10 compatible.
The best software for biological image analysis is Fiji
https://imagej.net/software/fiji/
You need to keep in mind that there is no such thing as red/green/blue pixels. All of the pixels have a range of intesities. You need to define what is green pixel for you in that range and what is not. For that you need to either manually select pieces of image of some colour and then count the number of pixels there, or define a threshold for the whole image that would separate pixels in each channel based on intensity distribution.
You can also try to find plugins for Fiji for leaf detection or maybe some standalone software.
Also, your images are far from perfect: too much shadow, overlit parts of leaves, labels, compression artifacts from JPG. If possible I would recommend to take new pictures of your samples. If your camera can do it, save in loseless image format: tiff or png.
You can try to join a free online course for plant image analysis
https://www.futurelearn.com/courses/introduction-to-image-analysis-for-plant-phenotyping
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