we are investigating a therapy for non-alcoholic fatty liver. On liver histology, the therapy seems to reduce steatosis. We want to support this finding by evaluating the amount of lipid accumulated in the liver but using paraffin-wax sections? Could you give me some suggestions?
Hi, paraffin sections stained with Hematoxylin-Eosin might be used for evaluating steatosis, of course it is a qualitative evaluation of percentage of steatosis according to NAS. We perform a quantitative analysis in fresh frozen section by staining with Oil-Red, then we measure the red area in ten optic fields per section. We validated that method by comparing its results with the lipid extract from the tissue. Other method is staining your sections with bodipy, I do not have experience using those dyes, but you might find it useful.
Best of luck
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