Yes, you can use SPR or BLI to determine the binding affinities and kinetics.

You can also estimate the avidity generated by your antibody-coated nanoparticle for your target.

The easiest way will be to immobilize your target by :

-amine coupling over the surface CM3 (SPR) or amine-reactive biosensors (ARG2 biosensors). Then you could inject at different concentrations your nanoparticule.

- biotin capture of your biotin-target using a SA sensorchip (SPR) or a Streptavidin biosensors (BLI). Then you could inject at different concentrations your nanoparticule.

You can perform also in the reverse way with the Immobilization of the nanoparticule over the surface or biosensor. I know that you can find papers where you can measure the affinities of glyco-nanoparticules using SPR technology.

Vanessa

Dear Vanessa! Thank you very much for your valuable input.

I Was trying with Blit’s ARG2 chip by immobilising my target protein And testing my Antibody coated NP. but I couldnt see any binding ranging from 200nM to 50 pM.

If you have any suggestions, help me out

Dear all, these two RG documents deal with similar situation. My Regards

https://www.researchgate.net/publication/274838123_Measuring_Binding_Kinetics_of_Antibody-Conjugated_Gold_Nanoparticles_with_Intact_Cells

https://www.researchgate.net/publication/264126271_How_Antibody_Surface_Coverage_on_Nanoparticles_Determines_the_Activity_and_Kinetics_of_Antigen_Capturing_for_Biosensing