We have been thinking to purify the plant compound by Ion exchange chromatography but one of our senior colleague suggested HPLC. So what would be the right choice?
Dear Jwala the reply a to question is in paper in this researcher that is my friend. The Bravo has worked with proteinase from latex at about 20 yers old. Im the lattes curriculum of Bravo when you coud to find references for to question.
I have worked with the protease from Euphorbia hirta latex and found no problem in purifying with anion exchange column, i guess You can go for Ion exchange....