Jwala Sivaccumar

3 Questions 2 Answers 0 Followers

Questions related from Jwala Sivaccumar

Problem within ShUFFLE strain in expressinv scfv ?

We recently trying to express scfv-his tagged through Cytoplasmic expressions using SHUFFLE strain. We are getting a high protein expression related to 50 kDa, instead of ~27 (our target). This...

19 December 2020 4,792 1 View

How to evaluate affinity of antibody -coated nanoparticle ? SPR/Blitz can help?

I Want to evaluate the binding affinity characterization and kinetic binding of the antibody coated on the nanoparticle. SPR, Blitz based experiments could help?

29 June 2020 8,661 3 View

Agent aiding ScFv solubilization?

I am producing some scfv through cytoplasmic expression system and so I use urea to retrive it from inclusion bodies. When i reduce the urea concentration, protein is getting precipitated. I am...

26 November 2018 588 0 View