Which tools are available to get the data on nucleotide change and rs number?

Hello everyone, My research work on to find out the novel and polymorphisms in selected gene.. I found few novel and SNP'S.. Now I am going to write a review article on SNP'S and their effect...

09 October 2019 7,962 3 View

How could I confirm my SNP's as a novel mutation?

06 July 2017 9,917 10 View

Could you suggest for my primers gradient temp. ?

Dear Sir, Please find find the attached file what are my primers and their details along with product length, few are previously optimized with human template standard 50ng DNA. Last 7 and 8...

05 June 2017 6,732 3 View

If you add DMSO in PCR mixture, will sequencing result getting noisy peaks?

Please give the suggestions to me. 10% DMSO added in PCR Master mixture and performed conventional PCR (The PCR product was gotten good intensity bands) and then performed Sanger sequencing....

05 June 2017 4,988 10 View

How can I avoid non specific bands while adding DMSO in pcr master mix? ?

05 June 2017 7,232 1 View

How can I get good PCR product with subject DNA samples?

First, I optimized the primers which were designed for specific exons, with different Temp (annealing Temp. of PCR) in gradient PCR with standard human blood DNA. After getting annealing Temp. did...

08 September 2016 7,887 6 View

Could anyone suggest for literature of PCR and their ?

suggestion for Literature of PCR, what ingredients, their concentrations with calculation of stock solution to working solution preparation for human genome (50-100 nano grams) amplification. Best...

06 July 2016 8,231 1 View

How to reduce non specific bands formation in PCR while standardizing the synthesized primers with different Temp.?

I designed primers for specific exons, the exons length are 3.6 kb, and 1.9 kb respectively. While standardizing the PCR with different temperature I did not find any band on gel. So again I...

05 June 2016 5,971 6 View

If DMSO added for amplification, will it give our specific product or other product?

When I optimized my designed primer on human standard DNA it worked and gives the selected exon/PCR product. but its using for subject samples few samples are getting PCR product and few samples...

01 January 1970 1,174 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

No title

01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View