07 July 2016 1 8K Report

suggestion for Literature of PCR, what ingredients, their concentrations with calculation of stock solution to working solution preparation for human genome (50-100 nano grams) amplification.

Best Regards,

Yedukondalu

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Could you suggest for my primers gradient temp. ?

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If you add DMSO in PCR mixture, will sequencing result getting noisy peaks?

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How can I avoid non specific bands while adding DMSO in pcr master mix? ?

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How can I get good PCR product with subject DNA samples?

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How to reduce non specific bands formation in PCR while standardizing the synthesized primers with different Temp.?

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If DMSO added for amplification, will it give our specific product or other product?

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