Which tools are available to get the data on nucleotide change and rs number?

Hello everyone, My research work on to find out the novel and polymorphisms in selected gene.. I found few novel and SNP'S.. Now I am going to write a review article on SNP'S and their effect...

09 October 2019 7,962 3 View

How to estimate the sample size for PhD by using Indian disease prevalence?

I am going to collect the human blood sample.\ I would like to know the sample size for my research work through our Indian disease prevalence. Actually in Indian prevalance is 1 in 2640, please...

06 July 2018 7,290 1 View

How could I confirm my SNP's as a novel mutation?

06 July 2017 9,917 10 View

Could you suggest for my primers gradient temp. ?

Dear Sir, Please find find the attached file what are my primers and their details along with product length, few are previously optimized with human template standard 50ng DNA. Last 7 and 8...

05 June 2017 6,732 3 View

If you add DMSO in PCR mixture, will sequencing result getting noisy peaks?

Please give the suggestions to me. 10% DMSO added in PCR Master mixture and performed conventional PCR (The PCR product was gotten good intensity bands) and then performed Sanger sequencing....

05 June 2017 4,988 10 View

How can I avoid non specific bands while adding DMSO in pcr master mix? ?

05 June 2017 7,232 1 View

How can I get good PCR product with subject DNA samples?

First, I optimized the primers which were designed for specific exons, with different Temp (annealing Temp. of PCR) in gradient PCR with standard human blood DNA. After getting annealing Temp. did...

08 September 2016 7,887 6 View

How to reduce non specific bands formation in PCR while standardizing the synthesized primers with different Temp.?

I designed primers for specific exons, the exons length are 3.6 kb, and 1.9 kb respectively. While standardizing the PCR with different temperature I did not find any band on gel. So again I...

05 June 2016 5,971 6 View

If DMSO added for amplification, will it give our specific product or other product?

When I optimized my designed primer on human standard DNA it worked and gives the selected exon/PCR product. but its using for subject samples few samples are getting PCR product and few samples...

01 January 1970 1,174 4 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View