Yedukondalu Kollati

10 Questions 11 Answers 0 Followers

Questions related from Yedukondalu Kollati

Which tools are available to get the data on nucleotide change and rs number?

Hello everyone, My research work on to find out the novel and polymorphisms in selected gene.. I found few novel and SNP'S.. Now I am going to write a review article on SNP'S and their effect...

10 October 2019 8,079 3 View

How to estimate the sample size for PhD by using Indian disease prevalence?

I am going to collect the human blood sample.\ I would like to know the sample size for my research work through our Indian disease prevalence. Actually in Indian prevalance is 1 in 2640, please...

07 July 2018 7,405 1 View

How could I confirm my SNP's as a novel mutation?

Dear Sir/Madam, I am working on DNA sequence (Sanger's method) to find the SNP's for my Project work. I suspected that one of the mutation is novel, how could I prove that as a novel mutation...

07 July 2017 10,027 10 View

Could you suggest for my primers gradient temp. ?

Dear Sir, Please find find the attached file what are my primers and their details along with product length, few are previously optimized with human template standard 50ng DNA. Last 7 and 8...

06 June 2017 6,858 3 View

If you add DMSO in PCR mixture, will sequencing result getting noisy peaks?

Please give the suggestions to me. 10% DMSO added in PCR Master mixture and performed conventional PCR (The PCR product was gotten good intensity bands) and then performed Sanger sequencing....

06 June 2017 5,113 10 View

How can I avoid non specific bands while adding DMSO in pcr master mix? ?

previous I optimised my designed primers without DMSO, but now my subject samples getting problem so I added DMSO, getting good intensity bands along with non specific bands. So now again...

06 June 2017 7,380 1 View

How can I get good PCR product with subject DNA samples?

First, I optimized the primers which were designed for specific exons, with different Temp (annealing Temp. of PCR) in gradient PCR with standard human blood DNA. After getting annealing Temp. did...

09 September 2016 8,005 6 View

Could anyone suggest for literature of PCR and their ?

suggestion for Literature of PCR, what ingredients, their concentrations with calculation of stock solution to working solution preparation for human genome (50-100 nano grams) amplification. Best...

07 July 2016 8,343 1 View

How to reduce non specific bands formation in PCR while standardizing the synthesized primers with different Temp.?

I designed primers for specific exons, the exons length are 3.6 kb, and 1.9 kb respectively. While standardizing the PCR with different temperature I did not find any band on gel. So again I...

06 June 2016 6,163 6 View

If DMSO added for amplification, will it give our specific product or other product?

When I optimized my designed primer on human standard DNA it worked and gives the selected exon/PCR product. but its using for subject samples few samples are getting PCR product and few samples...

01 January 1970 1,285 4 View