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Questions related from Yedukondalu Kollati
Hello everyone, My research work on to find out the novel and polymorphisms in selected gene.. I found few novel and SNP'S.. Now I am going to write a review article on SNP'S and their effect...
10 October 2019 8,076 3 View
I am going to collect the human blood sample.\ I would like to know the sample size for my research work through our Indian disease prevalence. Actually in Indian prevalance is 1 in 2640, please...
07 July 2018 7,402 1 View
Dear Sir/Madam, I am working on DNA sequence (Sanger's method) to find the SNP's for my Project work. I suspected that one of the mutation is novel, how could I prove that as a novel mutation...
07 July 2017 10,023 10 View
Dear Sir, Please find find the attached file what are my primers and their details along with product length, few are previously optimized with human template standard 50ng DNA. Last 7 and 8...
06 June 2017 6,854 3 View
Please give the suggestions to me. 10% DMSO added in PCR Master mixture and performed conventional PCR (The PCR product was gotten good intensity bands) and then performed Sanger sequencing....
06 June 2017 5,109 10 View
previous I optimised my designed primers without DMSO, but now my subject samples getting problem so I added DMSO, getting good intensity bands along with non specific bands. So now again...
06 June 2017 7,376 1 View
First, I optimized the primers which were designed for specific exons, with different Temp (annealing Temp. of PCR) in gradient PCR with standard human blood DNA. After getting annealing Temp. did...
09 September 2016 8,001 6 View
suggestion for Literature of PCR, what ingredients, their concentrations with calculation of stock solution to working solution preparation for human genome (50-100 nano grams) amplification. Best...
07 July 2016 8,339 1 View
I designed primers for specific exons, the exons length are 3.6 kb, and 1.9 kb respectively. While standardizing the PCR with different temperature I did not find any band on gel. So again I...
06 June 2016 6,158 6 View
When I optimized my designed primer on human standard DNA it worked and gives the selected exon/PCR product. but its using for subject samples few samples are getting PCR product and few samples...
01 January 1970 1,280 4 View