I am going to inject breast cancer (MDA-MB231) cells into female balb/c nude mice. I am injecting at the mammary fat pad. How would we ensure that the tumour is growing within or in the mammary fat pad?
Noah Sorrelle
I've found that, depending on where you inject, if your needle is subcutaneous, you're likely in the fat pad. I recommend doing a few practice injections with PBS + trypan blue or hematoxylin. After injecting, sack the mouse, and make sure the dye stayed in the fat pad. You'll find that if you're in the right spot, you'll rarely miss!
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