I have a exometabolites (yellow colour) in saline medium (containing 25 g/l NaCl). When I try to purify by using normal phase amino column the metabolites are retain into the column and I unable to elute. is there any method to elute this?
Remember, to use slightly acidified solvent (about 1 drop of HPLC grade Phosphoric Acid in 1 L will do) in order to break any hydrogen bonds between the stationary phase and your metabolite. Good luck!