How to edit dimensions of intake and exhaust valve in the SI engine (fueled with hydrogen gas)model build with GT Power software?

More Shirish Navale's questions See All

When we required crisper over homologous recombination ?

I am happily using homologous recombination for candida genetic experiments then suddenly a question came in my mind why some people use crisper and other methods for gentic methods and major...

15 November 2023 2,936 0 View

I did double restriction digestion of my plasmid with SacI and BAMH1 well 1,2,3,4 got multiple bands why???

why this multiple bands

20 October 2023 9,277 4 View

I did double restriction digestion of my plasmid with SacI and BAMH1 well 1,2,3,4 got multiple bands why???

Why I am getting multiple bands please help if anyone have any idea of this

20 October 2023 5,197 6 View

How gene disruption by Homologous recombination plates could give false colony?

In case of plasmid transformation gene vector could be empty for false colony but how in case of gene disruption based on homologous recombination can give false colony either antibiotic cassete...

17 October 2023 9,499 0 View

How do yeast cell and bacterial cells get oxygen in agar plates and packly closed 1.5 Mcts?

I was just thinking could it be possible just because 2- 5 hr storage of my yeast cells before soptting could switch to anaerobic or post diauxic shift

16 October 2023 4,955 1 View

Can we check gene disruption in yeast genome just by its colony pcr?

I have disrupted yeast gene in genome now I want to verify it without delaying it for overnight culture and then genome isolation so can I directly check it (gene disruption )from transformed...

06 October 2023 1,335 3 View

In colony pcr why we aspect only plasmid gene amplification not genomic gene is this right?

I have disrupted gene in E colli genome can check correct disruption in genome of e coli by colony pcr I had yet done colony pcr for plasmid gene amllification Or verification what about genome...

06 October 2023 3,556 2 View

Why I got unexpecred smear at end of this gel near 100 bp in double digestion of plasmid even in control?

I was done the 2hr restriction digestion of a plasmid but in gel run no fall out came instead a accumulation of something observed at bottom as in image what could be the possible reason

29 September 2023 7,101 2 View

Can we remove super coiling by heating ?

I had extracted dna from E. Colli which shown very low digestion even with Hf digestive enzyme It is suspected that becouse of 1-3 minute kept plasmid with Pd3 during plasmid Isolation caused...

13 September 2023 6,895 3 View

Can I use my pure target DNa sequence with pcr master mix for restriction digestion?

I had very few amount of my target Gene purified by gel extraction now to amplify gene I did pcr of the same.... so can I use this Pcr product directly use to my restriction digestion reaction.....

06 September 2023 9,565 1 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

In WECS : Gain values for conversion of PMSM rotor speed(rad/s) to p.u. input to wind turbine and Torque(pu) of WT to input of PMSM-Torque (Nm) ?

I am working on developing a MATLAB Simulink model of Wind turbine with PMSG followed by MPPT Boost converter. I have gone through so many literature and videos but I am not finding clear solution...

03 August 2024 888 1 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Rafael Lago Sari

Dear Shirish,

you need to look at the left side, where your valvecamconn template is located.

Clicking twice, will be possible to change the dimensions. In addition, you can delete the valve that you are using , find again in the library by pressing ctrl+H and paste in your model. All the dimensions will be empty, but you will can insert your valve dimensions

Duan Xiongbo

Hi, recently, I am calibrating GDI single cylinder engine by the GT Power, but it is difficult to calibrate the part load and the cylinder pressure is difficult to match the experimental data? what should I do?