Recently we came up with a sticky challenge; where by mistake my students dissolved LH-20 Sephadex in water for column preparation/packing buy not knowing that this will get dissolved. As Sephadex is an expensive material, we planned to dry it in Freeze drier, but after drying when we take it out from drier, it absorbs moisture from air and get sticky. We tried with rotary as well but get the same effect. After successive failures, we thought to dry it solvent - solvent extraction methodology. In this experiment, we dissolved LH-20 in distilled water, added equal volume of n-butanol in it and started its drying through rotary evaporator. As soon there is less than half of solvent left, we added equal amount of methanol it and dried it completely through rotary evaporator. Luckily, we get it dried with no stickiness and got a smooth powdered material.

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