Including every possible fluorescence, phosphorescence, vibrational relaxations and other transitions, I need to draw a Jablonski diagram. I know this can be done in ChemDraw. However, I use Linux.
I interest.
Hello everyone, I am using Quantum ESPRESSO software to calculate the total charge density of the compounds. However, I am unable to calculate the charge densities of valence band maximum and...
06 June 2024 4,498 4 View
How YOLOv8 is used to obtain the contours/object and calculate individual area
02 May 2024 5,396 3 View
I am optimizing a small organic molecule in a triplet second excited state using TD-DFT in ORCA 5.0. It is giving error termination in SCF gradient. How to get rid of it? Check the error below....
30 April 2024 6,806 6 View
Hi all, I want to have 301 copies/ul for my downstream experiments. The sample I am using is a purified plasmid, of which I know the concentration (ng/ul), volume (ul), and molarity(g/mol). How...
20 April 2024 2,468 4 View
Please explain the importance of : 1. DOE - Design Of Experiment 2. Statistical Testing Process Document
12 March 2024 1,556 1 View
I have bought strain B. braunii UTEX572 for my work, however, I do not see any positive growth after reviving it in both Bold 3N liquid and agar media supplemented with 10g/L glucose. Is there any...
10 March 2024 4,196 0 View
A person has very little thirst, dry mouth. If he drinks water, the water is coming out through the urine, but his body lacks water. Why does this happen and what is the remedy?
16 February 2024 1,255 3 View
I need some suggestions on working with the drug 5-fluoroindole. I am working on green microalgae and trying to make my strain tryptophan auxotroph. However, I am unable to make proper drug plates...
14 February 2024 1,013 1 View
When CRISPR-Cas9 RNP-based gene knock-in is performed in green microalgae, the donor cassette will contain the GOI and some selection marker genes. Post the gene integration how do I remove the...
04 February 2024 6,667 1 View
Due to global climate change, it has been observed that there is un-seasonal rainfall. Hence it is very much required to ascertain the change point in rainfall pattern as well as shifting in rainy...
29 January 2024 1,767 5 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...
29 July 2024 9,572 3 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View
what all details we will get from fluorescence life time data
10 July 2024 505 1 View
I am a beginner in this field. While I am reading papers and the understanding is getting better. However can someone explain in easy and simple words. Really thankful !
08 July 2024 8,337 5 View