Let me know whether the DOI issued by the research gate will be accepted by the anna university?

As per the rules and regulation DOI is needed and paper has to be opened in online by the anna university rules let me know whether the published article in some journal and the DOI issued by...

27 November 2020 7,540 2 View

What are the parameters to assess the effectiveness of online teaching?

Assessment of online teaching

22 October 2020 2,897 23 View

Can anyone suggest a way to overcome recombination when amplifying a pcDNA3 plasmid containing human SCN9A CDS insert?

I need to amplify a pcDNA3 plasmid containing human SCN9A CDS insert (pcDNA3-SCN9A construct). The CDS is around 6 kb, and the total construct length is approximately 11.2 kb, which is a bit...

07 June 2020 2,134 2 View

What are the essential areas in life long learning?

Areas in life long learning

31 December 2019 9,934 5 View

Does anyone have the Grass Instruments/AstroMed S88X Stimulator Manual?

Hello Everyone, Does anyone by chance have a Grass Instruments/Astro Med S88X stimulator manual? (http://www.neurolabparts.com/stimulators/99-grass-astro-med-stimulators-model-s88x.html). I...

17 December 2019 1,447 1 View

How to develop MCF7 mouse xenografts?

Hello everyone, I have two questions, please shed some light on this. I am new to in vivo studies and some may find my questions are trivial. TIA. My first question is, I wanted to develop the...

13 October 2019 1,208 2 View

I am interested to work in Image processing with automation application, looking for tools and resources if any?

Image processing with automation application

11 August 2019 1,945 3 View

Looking for opensource tool for ReaxFF simulation. If there something other than paid software ADF please reply ?

Looking for opensource tool for ReaxFF simulation. If there is something other than paid software ADF please reply ?

24 January 2018 6,596 5 View

How can we calculate the series resistance and shunt resistance from J-V curve?

Which method is easier to calculate the series resistance and shunt resistance from the J-V curve measurements?Is there any softwares to get the values directly?

10 January 2018 1,139 1 View

Is it necessary to synthesis second strand cDNA to amplify the gene of interest?

Dear All, I have synthesised cDNA first strand using SuperScript III (Invitrogen) and would like to amplify my gene of interest for overexpression studies? Is it necessary to synthesis second...

08 November 2017 5,895 11 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

How to find statistical data of EEG signals?

Hi all, I hope everyone is doing good. I am working on Machine Learning, I am working on EEG data for which I have to extract statistical features of the data. Using mne library I have extracted...

29 January 2021 6,686 1 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

Is PV array not available in MATLAb 2020a simulink?

I have MATLAB 2020a. PV array is not present in the simulink library. Can anyone please explain if the name of module has been changed or is it not available anymore? Is there any way to add PV...

24 January 2021 7,962 5 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

No title

13 January 2021 2,487 1 View

IET Digital Library Access?

Hello to everyone, I do not have access to the IET Digital Library at the institution where I work. Can those who have access to this library send me the publication whose DOI is given below?...

13 January 2021 9,385 2 View