Hello

You must first determine what you want to measure.

Do you want to use different methods in endodontic regenerative?

Yes, comparison between different scaffolds in endodontic regeneration 4 groups

In your study, you’ve 2 variables ( regenerative technique & scaffold type) . So, you can use 2 way Annova test. Try to download G*power software , it’s really very helpful tool to determine sample size of ur study. Additionally, you‘ve to read clinical trials dealing w/ the same methodology so you can have the same number. You may have higher number but not less. moreover, never to u underestimate the patient loss, so u’ve to add 10% of patients to compensate the drop out .

good luck dear