Is it possible(or logical) to notice an elevated autophagy using LC3-II based immunoblotting, but no difference by using GFP-LC3 based imaging study?

For example, I treat cells with "X" - performed immunoblotting for LC3-II - found elevated autophagy, then did the same treatment in GFP-LC3 transfected cells followed by confocal imaging - found...

06 April 2020 4,332 4 View

Can any one help to get complete bandwidth from 2 to 12 GHz which was mentioned in this paper using HFSS?

can any one help to get complete from 2 to 12 GHz bandwidth which was mentioned in this paper using HFSS... I got 5 to 12 GHz bandwidth Paper Title: Small Square Monopole Antenna With Inverted...

17 September 2019 3,508 3 View

How to validate the researcher as an instrument in qualitative research?

I am post doctoral student conducting a qualitative research. One of my thesis correction requirement was for me to state how I validate the researcher as an instrument since the researcher is...

24 December 2018 787 3 View

Example survey question for measuring efficiency in textile production unit?

creating a survey questionnaire for my research project.

02 September 2018 3,352 1 View

How variation in ph values affect quality of organic single crystals?

Variation in ph values of saturated solution of organic materials using a given solvent affects its quality. For example, inclusion-free, transparent, bulk size single crystals.

28 November 2017 949 2 View

Dear All, How to extract intronic sequences present in intron retained transcripts of RNA seq data?

I am working on RNA seq data (For banana plant) and we predicted that Intron-retention ( Alternative splicing) is predominately occurred. However, i need to extract intronic sequences from these...

26 July 2017 7,451 11 View

Does anyone have knowledge on HOMER - Renewable energy modeling?

How can one get the load demand data for importing the same in a HOMER software for renewable energy modeling? Can we use random data or we have to monitor it for a year or else do we have to get...

02 July 2014 495 8 View

I am facing a problem in binding and elution of an MBP tagged recombinant protein from MBP column. Can anyone lend their expertise?

I use 5X 0.5M HEPES buffer and 100mM ZnSo4 Nacl buffer into 1x as column buffer with pH 7.4 and 1X column buffer with 0.5M maltose as an elution buffer. Please suggest how I can overcome this...

15 April 2014 8,300 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View