Is it possible(or logical) to notice an elevated autophagy using LC3-II based immunoblotting, but no difference by using GFP-LC3 based imaging study?

For example, I treat cells with "X" - performed immunoblotting for LC3-II - found elevated autophagy, then did the same treatment in GFP-LC3 transfected cells followed by confocal imaging - found...

06 April 2020 4,332 4 View

How to validate the researcher as an instrument in qualitative research?

I am post doctoral student conducting a qualitative research. One of my thesis correction requirement was for me to state how I validate the researcher as an instrument since the researcher is...

24 December 2018 787 3 View

Example survey question for measuring efficiency in textile production unit?

creating a survey questionnaire for my research project.

02 September 2018 3,352 1 View

How variation in ph values affect quality of organic single crystals?

Variation in ph values of saturated solution of organic materials using a given solvent affects its quality. For example, inclusion-free, transparent, bulk size single crystals.

28 November 2017 949 2 View

Dear All, How to extract intronic sequences present in intron retained transcripts of RNA seq data?

I am working on RNA seq data (For banana plant) and we predicted that Intron-retention ( Alternative splicing) is predominately occurred. However, i need to extract intronic sequences from these...

26 July 2017 7,451 11 View

Does anyone have knowledge on HOMER - Renewable energy modeling?

How can one get the load demand data for importing the same in a HOMER software for renewable energy modeling? Can we use random data or we have to monitor it for a year or else do we have to get...

02 July 2014 495 8 View

I am facing a problem in binding and elution of an MBP tagged recombinant protein from MBP column. Can anyone lend their expertise?

I use 5X 0.5M HEPES buffer and 100mM ZnSo4 Nacl buffer into 1x as column buffer with pH 7.4 and 1X column buffer with 0.5M maltose as an elution buffer. Please suggest how I can overcome this...

15 April 2014 8,300 2 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View