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Questions related from Muthusamy Muthusamy
Dear Researchers! The attached images are stably expressing GFP fused target proteins in Brassica rapa young seedlings (Note: leaves were treated with Clearsee solution prior to imaging, to...
13 August 2021 8,728 6 View
Hi all Actually, I need chromosome start and end positions (10 chromosomes/Brassica rapa) and I have genome sequences in fasta file format. In the file, for each chromosome, it is mentioned like...
20 November 2020 2,709 4 View
Dear All Actually i want to know where and how lysine is binding in a lysine feedback negative regulating enzyme, apartate kinase in plant system. I have tried some online tools but it is not...
11 February 2019 8,442 8 View
Hi all Actually i have to design sgRNA using CRISPRseek and screen the whole genome for off-target analysis. Unfortunately, for banana plants BS genome Packages is not available with Bioconductor...
13 April 2018 7,118 2 View
I am working on RNA seq data (For banana plant) and we predicted that Intron-retention ( Alternative splicing) is predominately occurred. However, i need to extract intronic sequences from these...
27 July 2017 7,545 11 View
I am planning to use pCAMBIA 1305.1 vector for Agrobacterium mediated transformation for my plants. 1. I just want to know, whether having GUS as reported gene will have any undesirable effect on...
15 December 2016 7,264 8 View
Actually i have to find out the type of Alternative splicing eg. exon skipping, from the transcriptome data. I am not familiar with R packages and linux system. Earlier i was using ASFinder and...
09 November 2015 380 5 View
