I am working on expression of 23 different markers in ILCs in wt mice. To analyze the data, I run FlowSOM. As you may know, the FlowSOM gives the heat map for markers expression. Since Some of the markers are highly expressed and some have low level of expression, I need to do a normalization before running FlowSOM. Is it possible to normalize the sample in FlowJo? Since I don’t have any control samples, how should I perform normalization? I am wondering if you know any method that could help me?
Thank you so much.
Dear Mehrnoush
To do this, I standardized the data using the Spss application. Add your data in Spss than go to Analyse, Descriptives, and mark save standardized values.
This my article that has two pHeat maps.
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Mehdi Babaei
Dear Mehdi,
Thank you for the reply. I can not use Spss since the data is flow cytrometry data.
Best Regards