Hello everyone,

I have been using a commercial available scaffold to develop a 3D model where fibroblasts are seeded in the scaffold but keratinocytes are seeded on top to develop epidermal layers (not connected to the scaffold anymore). For some time now we have been observing that during the histology/immunofluorescence staining process that these layers seem to be washed away.

Have you ever encountered this problem? If yes, what has helped you?

Thank you!