How to distinguish two or more species having same mass in a Quadrupole Mass Analyzer (QMA)?

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In Chip-seq, what will be the impact of adding proteinase K before removing the magnetic beads?

I was following this chip-seq protocol https://www.cellsignal.com/products/chip-kits-reagents/plus-sonication-chromatin-ip-kit/56383 what will be the consequence if on the following state "VI....

20 July 2024 5,612 0 View

Researchers, if asked, which 5 Indian Gangster movies would you rate highly or recommend?

Why do I not try one on a Gangster or Mafia movie(s)? This is what I have been thinking for a month or so. Finished the one on ED (Emily Dickinson), and submitted it to a journal.

21 June 2024 2,717 2 View

Would you please share the index of first lines / poem index of the book 100 Selected Poems: Emily Dickinson?

I do have "The Complete Poems of Emily Dickinson."

01 January 2024 5,297 0 View

Will the universe expand forever?

We know that the universe is expanding with acceleration. Can there be any event of such a large scale that can alter the trend of expansion and begin contraction or even stagnant universe?? Lets...

25 December 2023 8,898 3 View

When we add template in a ready to use master mix for PCR amplification, the sample/template get diluted?

I used a established PCR kit to amplify the target DNA. The instructions from kits was given to use (1.5-15ng/ul) concentration of DNA. After DNA extraction procedure i got 23ng/ul;then i adjust...

13 November 2023 4,699 4 View

Any protocol set for fish ethology study with different parameters?

Hi! I am quite a novice in the field of fish ethological research. I am looking for a step-wise guide for parameter choice and data analysis. Especially the movement tracking and behavioural...

08 October 2023 7,282 3 View

Researchers, do you by any chance have any soft copy of the book 𝘓𝘦𝘵𝘵𝘦𝘳𝘴 𝘰𝘧 𝘌𝘮𝘪𝘭𝘺 𝘋𝘪𝘤𝘬𝘪𝘯𝘴𝘰𝘯?

𝙇𝙚𝙩𝙩𝙚𝙧𝙨 𝙤𝙛 𝙀𝙢𝙞𝙡𝙮 𝘿𝙞𝙘𝙠𝙞𝙣𝙨𝙤𝙣 by Emily Dickinson (Author), Mabel Loomis Todd (Editor)

28 September 2023 6,709 1 View

May I get various observational evidences about the existence of diverse (nucleus) acoustic waves in the interiors of compact astrophysical objects?

Dear Colleagues, I would like to request various observational evidences about the existence of diverse (nucleus) acoustic waves in the interiors of compact astrophysical objects, such as white...

17 August 2023 3,363 0 View

Simulation of Transient Analysis of Tunnel FET in Sentaurus?

Can anyone please help in regarding the inverter based on TFET device? I am facing an issue in SDEVICE. I have used the mixed mode code for inverter from manual and used the Tunnel FET physics. I...

25 May 2023 8,583 1 View

Which softwares are better to draw schematic diagrams of beams, plates etc?

I want to know which software is used by authors to draw schematic diagrams of beams, plates etc. for there articles.

22 May 2023 7,631 6 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Hello dear researchers. I would like to know what is a normalized mass in an EDX analysis?

EDX analisys

31 July 2024 5,947 2 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

Could international experts in media and journalism, AI, communications and international communication share this conference link?

"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/

23 July 2024 3,139 6 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View

Hadi Jabbar Alagealy

mass analyzer separates ions based on the stability of their flight trajectories through an oscillating electric field in the quadrupole.

Soumik Kumar Kundu

Thank you Hadi Jabbar Alagealy sir for answering. I am curious, is it possible of getting a peak of radioactive element in a newly purchased QMA? Or it is some other isotope element with same mass of a radioactive element?