How to distinguish the nonspecific background from the Endogenous alkaline phosphorylase in DIG ISH of frozen section?

I want to get a clear background DIG-ISH. I cloud trust that the DIG- riboprobes are right, and they are not degrdation about my riboprobes. I couldn't confirm it is no signal or a endogenous...

24 March 2023 9,885 0 View